Fisher scientific illkirch
WebMay 6, 2024 · Protein concentration of antibody stock solution (PBS 1/20×, pH 7.5) was determined by UV absorbance using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Illkirch, France). Webinstructions (Ambion MaxiScript SP6/T7 kit, Thermo Fisher Scientific, Illkirch, France). The resulting RNA transcripts were processed using 2 µL of DNase I (2 units/µL) for 15 min at 37°C, followed by a final purification step (RNeasy mini kit, Qiagen, Courtaboeuf, France). The RNA concentration was determined (Qubit fluorom-
Fisher scientific illkirch
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WebMar 31, 2024 · Spodoptera frugiperda 9 (Sf9) cells (ATCC CRL-1711) were maintained in SF-900TM II SFM (Thermo Fisher Scientific, Illkirch, France) at 28°C. Seventy-two hours before transfection, the cells were plated on a glass coverslip coated with poly D lysine (0.1 mg/ml) in 35 mm Petri dishes. WebThermo Fisher Scientific Digital Sales in Illkirch, France. ... Thermo Fisher Scientific is an EEO/Affirmative Action Employer and does not discriminate on the basis of race, …
WebThermo Fisher Scientific (Illkirch-Graffenstaden) is committed to sending Zero Waste to Landfill and is dedicated to improving waste management on site in line with the Defra … WebMar 1, 2024 · 15 μl of the PCR reactions were loaded on a 2% Gelgreen (Biotium, California USA) stained agarose gel in 0.5X TAE buffer and separated by electrophoresis at 100 Volts. 1 μg of DNA ladder (GeneRuler 50bp, Fisher Scientific, Illkirch France) serves as a reference for the migration.
WebJan 4, 2024 · Hypersil GOLD NH 2 chromatographic Silica (5 µm) was kindly donated by Thermo Scientific (Illkirch-Graffenstaden, France). The bulbs of L. radiata were purchased from Lotta Plants (Haarlem, Holland). 2.2. AChE immobilization. ... °C freezer. The frozen bulb pieces were ground by Spex Certiprep™ Cryogenic Freezer/Mill™ (Fisher Scientific ... Fisher Scientific Parc d’Innovation BP 50111 67403 Illkirch Cedex +33 (0) 3 88 67 53 27 [email protected] fr.fishersci.com Germany Fisher Scientific GmbH Im Heiligen Feld 17 D-58239 Schwerte +49 (0) 2304 932 5 Fax: +49 (0) 2304 932 950 [email protected] de.fishersci.com United Kingdom Fisher Scientific UK Bishop Meadow Road
WebApr 29, 2024 · About 300 . × 10 6 cells were collected on days 2, 4 and 7 on a GF/C glass fibre filter (Whatman, Fisher Scientific, Illkirch, France). Total lipids were extracted in a chloroform/methanol/0.28% NaCl (1/2/0.2, v/v/v) mixture (Bligh & Dyer, Citation 1959). The solvent was then evaporated under partial vacuum using a rotary evaporator (Heidolph ...
WebApr 10, 2024 · To prevent RNA degradation during thawing, RNA Later Ice (Fisher Scientific, Illkirch, France) was added before thawing for samples collected in 2013. In 2014 and 2015, samples were directly ... ray hughes soundWebThe Fisher Scientific Channel is building a bridge between business and science as the single source for a comprehensive range of laboratory equipment, chemicals, … simple validation form in javascriptWebJul 13, 2024 · The pellet was resuspended in PBS and cells were centrifuged at 1900 rpm for 5 minutes. Finally, the PBMC pellet was frozen in a medium containing 90% of Fetal Bovine Serum (FBS) (Gibco, Thermo Fisher scientific, Illkirch, France) and 10% of dimethyl sulfoxide (DMSO) (Sigma Aldrich, St. Quentin Fallavier, France). ray hughes symphony of heavenWebMar 17, 2024 · Then, cells were rinsed for 5 min with 0.1N HCl (pH 1, Fisher Scientific, Illkirch, France) or with 3M acetic acid (pH 2.5, Fisher Scientific, Illkirch, France) in order to decrease pH and reveal sulfated and non-sulfated glycosaminoglycans, respectively. Then, cells were stained with 1% Alcian Blue (8GX, Sigma-Aldrich, Saint Quentin … ray hughes sermonsWebIllkirch, Alsace 50111, FR Get directions Bishop Meadow Road Loughborough, England LE11 5RG, GB ... Connect with the Fisher Scientific team, which is part of Thermo Fisher Scientific, and learn ... simple valentine craft ideas for kidsWebNov 23, 2024 · To this end, the preincubated stool samples were diluted from 10 −1 to 10 −10 in phosphate buffer saline (Thermo Fisher Scientific, Illkirch, France) to dilute the sample and identify the maximum number of bacterial species. Then, 50 µl of diluted sample was deposed and homogenized on blood agar plates (Biomerieux, Marcy l’Etoile, France). simple value stream mapping examplesWebFisherbrand™ Superfrost™ Plus Microscope Slides. Boekel Scientific™ Large Paraffin Wax Dispenser. Invitrogen™ RNAqueous™ Total RNA Isolation Kit. Brady™ BBP™ 35 Multicolor Sign and Label Printer. VeriSpec™ pH Buffer, Certified Reference Material. Fisherbrand™ Vinyl Chair - Medium Bench Height with Chrome Foot Ring and Casters ... simple value me48113ff fridge freezer - white