Phospho tbk1 antibody

Author: bmsu

August undefined, 2024

WebDec 10, 2015 · Immunofluorescence experiments using a phospho-TBK1 (pS-172) specific antibody on A549, H1650, Calu-6 and PC9 non-small cell lung cancer (NSCLC) cell lines as well as the immortalized human tracheobronchial epithelial cell line AALE established that phospho-TBK1 localized to centrosomal regions during prophase and prometaphase, … WebJul 7, 2024 · Colocalization of DNA with ubiquitin and phospho-TBK1 was also defective in Gt and ∆CTT cells but was normal in ... Cells were stained with the following antibodies: APC CD11b (Biolegend ...

Phospho-TBK1 (Ser172) Polyclonal Antibody - Thermo Fisher Scientific

WebMonoclonal antibody is prepared from animals immunized with a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human TBK1. Validations. Biological … WebDec 10, 2015 · Phospho-TBK1 and total TBK1 were also present in additional fractions; correlating with the observation that phospho-TBK1 also is associated with spindle … northern irish assembly elections 2017

Human-Reactive STING Pathway Antibody Sampler Kit

WebPhospho-TBK1/NAK-S172 Rabbit mAb (AP1026) Western blot analysis of extracts of various cell lines, using Phospho-TBK1/NAK-S172 Rabbit mAb (AP1026) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were … WebThe Phospho-TBK1 (Ser172) assay measures TBK1 when phosphorylated at Ser172. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-TBK1 (Ser172) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. WebProduct Details Antibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: T2K, NAK Reactivity: Human (QC Testing) Isotype: Mouse IgG1, κ Immunogen: Human TBK1 C terminus Recombinant Protein Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: AB_1645537 northern irish banknotes

Phosphorylation of innate immune adaptor proteins MAVS, STING, and …

WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: … WebHere we show that STING stimulates IRF3 phosphorylation by the kinase TBK1 in an in vitro reconstitution system. Using this system, we identified a carboxyl terminal region of STING that is both necessary and sufficient to activate TBK1 and stimulate IRF3 phosphorylation. northern irish borderWebMay 6, 2024 · Finally, TBK1 overexpression and knockdown in Drosophila can reciprocally increase and decrease tau-induced neurodegeneration. Together these data support a hypothesis that TBK1 activation may enhance tau phosphorylation and neuronal loss in AD and related tauopathies. Results Identification of novel tau-interacting kinases in AD brain how to roomate in berry ave

"WebRecombinant Anti-NAK/TBK1 (phospho S172) antibody [EPR2867 (2)] - BSA and Azide free (ab186469) Datasheet Certificate of Compliance Submit a review Submit a question References (1) $905 Product size 100 µg $905 1 mg $8,985.00 Add to basket Order now and get it on Thursday March 02, 2024 Custom quote Bulk request Shipping info " - Phospho tbk1 antibody

Phospho tbk1 antibody

Phospho-TBK1/NAK-S172 Rabbit mAb-Phospho …

WebRecently, a new IKK family member, TBK1 [ (TANK-binding kinase), also known as NAK and T2K] has been identified. TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner. Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. WebPhospho-TBK1 (Ser172) Antibody (PA5-105919) in IHC (P) Immunohistochemistry analysis of paraffin-embedded Phospho-TBK1 (Ser172) in mouse kidney tissue sections. …

Did you know?

WebDec 9, 2013 · TBK1 is phosphorylated in its activation loop, leading to IRF3/7 activation and Type I interferon (IFN) production. IPS-1 and STING localize to the mitochondria and endoplasmic reticulum,... WebJan 30, 2024 · TBK1 Antibody (A-6) is an IgG 1 κ mouse monoclonal TBK1 antibody (also designated NAK antibody, T2K antibody, Encephalopathy, acute, infection-induced, herpes-specific, 8 (IIAE8) antibody, or Frontotemporal dementia and/or amyotrophic lateral sclerosis 4 (FTDALS4) antibody) suitable for the detection of the TBK1 protein of mouse, rat and …

WebWestern blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml), up to 24h, using Phospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower). Show More WebWestern blot - Phospho-TBK1/NAK-S172 Rabbit pAb (AP0847) Western blot analysis of HeLa, using Phospho-TBK1/NAK-S172 antibody (AP0847) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit …

WebAnti-phospho-TBK1 (Ser172) Antibody 07-2192 Sigma-Aldrich Anti-phospho-TBK1 (Ser172) Antibody Download Zoom This phospho-TBK1 antibody is validated for use in WB for the … WebThe Phospho-Specific Mouse Anti-EGF Receptor pSer1047 ( VMA00746) and anti-total Rabbit Anti-EGF Receptor were incubated together. hFAB Rhodamine Anti-Tubulin was added with the fluorescent secondary antibodies. The fluorescent images were captured using the ChemiDoc MP Imaging System. 5 Phosphatase treatment

WebImmunogen: Phosphorylated Human TBK1 Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: AB_647214 Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% …

WebThis phospho-TBK1 antibody is validated for use in WB for the detection of the phospho-TBK1 protein. Quality Evaluated by Western Blot in EGF treated and untreated HEK293 cell … northern irish clothing brandsWebPhospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb (PE Conjugate) recognizes endogenous levels of TBK1 only when phosphorylated at Ser172. This antibody may … northern irish drivers licenceWebMonoclonal Antibody for studying TBK1 (Ser172) phosphate. Cited in 553 publications. Validated for WB, IP, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit Monoclonal Antibody (CST #5483) is ready to ship. how to roof ridge capWebThe Phospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb Antibody has been validated for the following applications: Precipitation, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blot. Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb detects endogenous levels of TBK1 only when phosphorylated … how to roof tiles installationWebOur TBK1 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit, Mouse and Goat. These antibodies have been verified by Knockdown, Knockout and Relative expression to confirm specificity to TBK1. Find the TBK1 antibody that fits your needs. how to roommate in berry avenueWebJan 17, 2024 · TBK1 Antibody (108A429) is an IgG1 κ mouse monoclonal TBK1 antibody (also designated TBK1 antibody or TANK binding kinase 1 antibody) suitable for the detection of TBK1 of mouse, rat and human origin by WB, IP and ELISA. ... Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive … how to roof videoWebWhile TBK1 is known to phosphorylate cargo receptors to promote ubiquitin binding, it is unknown whether TBK1 phosphorylates other proteins to promote mitophagy. Using global quantitative proteomics, we identified S72 in RAB7A, a RAB previously linked with mitophagy, as a dynamic target of TBK1 upon mitochondrial depolarization. northern irish crime writers