Webb磷酸盐缓冲溶液 (PBS) 是一种平衡盐溶液,适用于各种细胞培养应用,例如解离前清洗细胞、运输细胞或组织样品、稀释细胞进行计数和制备试剂。 在细胞解离之前,需采用配方中不含钙和镁的制剂从培养物中冲洗掉螯合剂。 浓缩液形式的 Gibco™ PBS 在稀释时需要调整 pH(详见 实验方案 )。 我们针对大量细胞培养应用提供了各种 Gibco™ PBS 制剂 。 … WebbThe section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the KCNK6 antibody at a dilution of 1:100 for 30 minutes at room temperature.
Dry-blend Buffered Packs - Thermo Fisher Scientific
WebbFill the tube with cold PBS w/ 0.1% BSA + 0.6% Na-citrate (without Ca2+and Mg2+) and centrifuge at 300 x g at 2-8°C for 10 min. Discard the supernatant and resuspend the cells in 50 ml cold PBS w/ 0.1% BSA + 0.6% Na-citrate (without Ca2+and Mg2+). Centrifuge at 300 x g at 2-8°C for 10 min. Webb13 apr. 2024 · L. donovani promastigotes were incubated with PBS containing 50 µg/mL CFSE at room temperature for 30 min, then washed with RPMI 1640 three times. 2.2. Measurement of Soluble SIRPα Serum levels of soluble SIRPα in naïve or L. donovani -infected BALB/cA mice were measured with a Mouse SIRPα ELISA kit (RayBiotech, … tara heights school papillion ne
PBS, pH 7.4 - Thermo Fisher Scientific
WebbPhosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cellular applications, such as washing cells/tissue during immunohistochemistry (IHC), immunocytochemistry (ICC); transporting cells or tissue; diluting cells for counting; and preparing reagents. PBS is formulated without calcium and magnesium. WebbThermo Scientific StartingBlock Blocking Buffers are PBS or TBS formulations of a proprietary protein that provides exceptional performance and broad compatibility with membrane (Western blot) and … WebbInvitrogen Anti-CCL5 (RANTES) Polyclonal, PeproTech®, Catalog # 500-P78-1MG. Tested in Western Blot (WB) and ELISA (ELISA) applications. This antibody reacts with Rat samples. Supplied as 1 mg purified antibody. tara heights nashua unit 5 211